Terminal Deoxynucleotidyl Transferase (TdT) Buffer
Description
Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is a DNA polymerase that catalyzes the addition of deoxynucleotides to the 3fi hydroxyl terminus of DNA. A TdT Technical Bulletin is available.Applications: Homopolymer tailing of vector and insert for cloning. Labeling oligonucleotides with biotin (1,2), 32P- or 35S-label (3), or in apoptosis (TUNEL) (4,5).
Source: Purified from a baculovirus clone of calf thymus TdT.
Performance and Quality Testing: Endonuclease, 3´ and 5´ exodeoxyribonuclease, and levels of incorporation tested.
Unit Definition: One unit incorporates 1 nmol dATP into acid-precipitable material in 1 h at 37°C, using d(pA)50 as a primer.
Hazard Warning: Toxic; potassium cacodylate contained in reaction buffer. Also contains cobalt chloride, a highly toxic chemical.
See MSDS.
Unit Reaction Conditions: 0.2 M potassium cacodylate (pH 7.2), 10 mM MgO4 C4 H6 , 1 mM 2-mercaptoethanol, 0.5 mg/ml BSA,
100 flM d(pA)50, 1 mM [3H]dATP, and enzyme in 0.15 ml for 1 h at 37°C.
Specifications
Product Size: | 2 x 0.8 mL |
Shipping Condition: | Approved for shipment on Wet or Dry Ice |
Contents & storage
- Terminal Deoxynucleotidyl Transferase, Recombinant (rTdT) is supplied with vial of 5X buffer [500 mM potassium cacodylate (pH 7.2), 10 mM CoCl2 , 1 mM DTT]. Store at -20°C.
Note:
Pembelian bahan Kimia ini harus melampirkan Company Profile (CV atau PT), Perorangan tanpa Identitas perusahaan tidak akan dilayani.
#tokokimiabatam, #bahankimia, #kimiabatam, #chemicalsbatam, #cleaningchemicals, #floorcaremaintenance, #floorchemicals, #floorfinish, #alkalinecleaner, #superkleen
Post a Comment
Note: Only a member of this blog may post a comment.