Viral Production Cells

Description

Gibco Viral Production Cells are a derivative of the HEK 293F cell line, and are a core component of the LV-MAX Lentiviral Production System. Viral Production Cells have been adapted to a special chemically-defined, serum-free and protein-free LV-MAX Production Medium. They are maintained in suspension culture and will grow to high density in LV-MAX Production Medium. Viral Production Cells are highly transfectable by LV-MAX transfection reagent and generate superior lentiviral production compared to polyethyleneimine (PEI)-based lentiviral production methods.

• Suspension culture cells facilitate high-throughput screening or scale-up
• Higher viral production levels than other suspension HEK 293 cells
• Rapid cell growth and high culture viabilities

Frozen cells are supplied in a vial containing 1 mL of cells at 1 x 107 viable cells/mL in LV-MAX Production Medium and 10% DMSO. The cells should be thawed directly into LV-MAX Production Medium.

The cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable in volume from 96-deep well blocks to 2-L flasks and bioreactors. Lentiviral vector harvest is typically performed at two days post-transfection.

Specifications

Cell Line:	Viral Production Cell-Line
Cell Type:	Kidney (Embryonic)
Culture Type:	Suspension Cell Culture
Media Adaptation:	LV-MAX™ Production Medium
Number of Cells:	1 x 10⁷
Product Size:	1 mL
Shipping Condition:	Dry Ice

Contents & storage

Store in liquid nitrogen until ready to use. Do not store the cells at -80°C.

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